FIGURE 11.

Effects of benzalkonium (BAK) in experimental models on trabecular cell apoptosis. Left, Immunostaining using the TUNEL technique for apoptotic cells in the trabecular meshwork (TM) and iris root after BAK injection. Staining of apoptotic cells appears in green in the nuclei. Numerous cells are stained in the iridocorneal angle structures. Nuclei are counterstained in blue by DAPI. The cornea, limbus, and conjunctiva are visible on the right, the iris root and ciliary body at bottom left, and TM in between. Middle, Higher magnification of the TM containing many green-stained apoptotic cells. Right, Control eye subconjunctivally injected only with saline: no green staining of apoptotic cells in the anterior segment (ciliary processes are clearly visible at bottom right, cornea on left, and iris in between). Bars = 100 μm.