FIGURE 5.

Assessment of fractalkine in trabecular meshwork cells submitted to benzalkonium (BAK) or TNF-α. Indirect immunostaining for fractalkine (in red) in trabecular cells in vitro (nuclei are counterstained in blue by DAPI). Top left, Low, almost negative fluorescence intensity in basal condition. Top right, Strong expression following TNF-α. Bottom left, BAK 5×10⁻⁵% stimulation for 24 hours. Bottom right, 5×10⁴% stimulation for 24 hours. After BAK stimulations, cells lost their elongated form and cell density was reduced. Bars = 50 μm.