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Assessment of fractalkine receptor CX3CR1 in trabecular meshwork cells submitted to benzalkonium (BAK) or TNF-α. Indirect immunostaining for CX3CR1 (in red) in trabecular cells (nuclei in blue). Top left, Negative fluorescence intensity in basal conditions. Top right, Increased expression after TNF-α. Bottom left, BAK 5×10−5%. Bottom right, BAK 5×10−4% stimulations for 24 hours. Bars = 50 μm.
