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. 2012 Dec;110:40–63.

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Interactions between benzalkonium (BAK) and CXCL12. Left, Anti-apoptotic effect of CXCL12 on trabecular meshwork cells exposed to a toxic dose of BAK. Apoptosis was measured using the Hoechst test in cold light cytofluorimetry. Apoptosis was normalized after reporting Hoechst over the neutral red signal; **P<.05 (ANOVA). Middle, Effects of BAK on CXCL12 expression in TM cells assessed by flow cytometry. P <.001 for all BAK stimulations compared to the control (ANOVA). Right, Effect of BAK on CXCR4 expression assessed by flow cytometry. P<.001 for 0.0001% and 0.001% BAK stimulations (ANOVA); not significant for BAK 0.01%. MFI, mean fluorescence intensity. Bars = Standard Error.