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Confocal microscopy of 1-AZA-labeled tadpoles. (a) Bright-field image (4× magnification) of tadpole brain with 15 µM 1-AZA. (b) 4× fluorescent image of tadpole brain with 15 µM 1-AZA. (c) Overlay of fluorescent and bright-field images. Scale bar = 200 µm.