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EpoD effect on neurosteroid allopregnanolone (ANS) dose response in tadpoles. EpoD (2 µM) lowered tadpole immobilization from 34 ± 20% to 10 ± 7% in the presence of 3 µM allopregnanolone (t test, P(T≤t) two-tail = 0.036) and from 61 ± 9% to 50 ± 10% (t test, P(T≤t) two-tail = 0.179) in the presence of 4 µM allopregnanolone after 3 h incubation in pond water with 0.5% ethanol, before switching to fresh water.
