Table I.
PCR conditions and primers used in the study.
| PCR conditions (°C/s) |
||||||||
|---|---|---|---|---|---|---|---|---|
| Gene/target | Primers | Sequence 5’– 3’ | Denaturation | Annealing | Extension | No. of cycles | Length of PCR product (bp) | References |
| 18S rRNA gene/ | RIB19 | CGGGATCCAACCTGGTTGATCCTGC | 92/60 | S4/60 | 72/120 | 30 | 1,700 | da Silveira et al. (2011) |
| Babesia/ | RIB20 | CCGAATTCCTTGTTACGACTTCTC | ||||||
| Theileria sp. | BabRumF | ACCTCACCAGGTCCAGACAG | 92/60 | 54/60 | 72/120 | 30 | 420 | |
| BabRumR | GTACAAAGGGCAGGGACGTA | |||||||
| pCS20 gene /E. | AB 128 | ACTAGTAGAAATTGCACAATCTAT | 94/60 | 55/60 | 72/120 | 4S | 279 | Mahan et al. (1992) |
| ruminantium | AB 129 | TGATAACTIGGTGCGGGAAATCCTT | ||||||
| msp4 gene/A. | MSP 4S | GGGAGCTCCTATGAATTACAGAGAATTGTTTAC | 94/30 | 60/30 | 68/60 | 40 | 8S1 | de la Fuente et al. (2001) |
| marginale/ovis | MSP 43 | CCCCGGATCCTTAGCTGAACAGGAATCTTGC | ||||||