Fig. 1.

Western blots of endogenous transient receptor potential (TRP) M2 isoforms expressed in adrenal glands and neuroblastoma. Whole cell lysates from adrenal gland tissues and neuroblastoma were isolated, and 200 μg of protein were loaded in each lane. Western blots were probed with anti-TRPM2-C antibody (A) to detect TRPM2-L (171 kDa) and then with anti-TRPM2-N antibody (B) to detect TRPM2-L and TRPM2-S (95 kDa). GAPDH was probed to determine equivalent loading. Representative blots with 3 adrenal (A) and 3 neuroblastoma (N) samples probed with anti-TRPM2-C (A), anti-TRPM2-N (B), and GAPDH (A and B) are shown. GAPDH loading control was the same for A and B. Bands were quantified by densitometry, and relative expression of TRPM2/GAPDH for neuroblastoma, normalized to mean adrenal gland/GAPDH on the same blot, was determined. Statistical differences between adrenal gland tissue (13 samples) and neuroblastoma (17 tumor samples in A and 16 in B) were analyzed using Student's t-test: *P ≤ 0.01.