Table 1.

Primer sequence used for qPCR analysis of glucose transporter isoforms in VSMCs

Gene	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Product Size, bp
GLUT1	CTGCTCATCAACCGCAAC	CTTCTTCTCCCGCATCATCT	129
GLUT2	TACATTGCGGACTTCTGTGG	AGACTTTCCTTTGGTTTCTGG	108
GLUT3	CAGCGAGACCCAGAGATG	TTGGAAAGAGCCGATTGTAG	233
GLUT4	TGGGCTTCTTCATCTTCACC	GTGCTGGGTTTCACCTCCT	121
GLUT5	CCTTTGGGTCATCCTTCCA	ACAGACCACAGCAACGTCAA	145
GLUT6	ATGCCCTGGTCTACACATCC	GAACACGGACCCAAACCA	104
GLUT7	TCGGTGCCTACAGTTTCATC	AATGCGGTTTATCTCCACAA	113
GLUT8	GCCAAGTTCAAGGACAGCA	GACCACACCTGACAAGACCA	129
GLUT9	CCTGTTTGGAGTGATTGTGGT	CTTGCCTCGTTGTGCTTCTC	105
GLUT10	TGGTCTTTGTCAGTGCCTTC	GCTCTTCCTCGTATCTCCAC	91
GLUT11	CGTGATGGGACAGGTGGT	GCTTTCAGGGAGCAGAGG	127
GLUT12	GGGCATCGTAAATCTCAACA	CCATAAATCACAGACTCATCCA	96
HMIT	GCAGCTCTCAGGCATTAACA	ACAAGCCAGACTCCCACAAG	147
GLUT14*	CCATGTGTCTGGAATTGGTG	AGCAACTGTGATGGCAAAGA	103
SGLT1	GTCATCTCCCTCCTCACCAA	TCCAGGTCAATACGCTCCTC	95
SGLT2	TACTTCGCCATTGTGCTGTT	CTCCCGTTCCTCCTTGCT	132
B2M	GGGTTTCATCCATCCGACA	ACACGGCAGGCATACTCATC	161
GAPDH	ACCCAGAAGACTGTGGATGG	AGTAGAGGCAGGGATGATGTT	84

qPCR, quantitative real-time PCR; VSMCs, vascular smooth muscle cells; GLUT, glucose transporter; SGLT, sodium-dependent glucose transporter; HMIT, H⁺/myo-inositol transporter; B2M, β₂-microglobulin. GLUT14

^*primer pair recognizes the long isoform of the transcript.