Skip to main content
. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: Oncogene. 2012 Jun 11;32(17):10.1038/onc.2012.234. doi: 10.1038/onc.2012.234

Figure 3. Decreased contractility enhances McTNs.

Figure 3

Figure 3

Figure 3

Figure 3

A. McTNs increase in cells suspended in 50µM blebbistatin (p<0.05), but are suppressed in cells suspended in 20µM ML-7 (p<0.001). B. Suspended cell immunofluorescence of MCF-10A cells and a representative PTEN−/− clone in the presence of 50µM blebbistatin or C. 20 µM ML-7. Induced McTNs contain α-tubulin (green) extending under the actin (red) cortex. Cells treated with ML-7 show no outwardly extending protrusions (bar = 10µM). D. Real time attachment measurements of blebbistatin treated MCF-10A and PTEN−/− cells attach approximately 2-fold quicker at 1 hour compared to the DMSO control cells. At all time points, ML-7 treated cells attached approximately 50% less than that of the controls. Impedance values are normalized to DMSO controls (n=4, representative results from triplicate experiments).