Table 1.

Brief introduction of network pharmacological technologies methods and tools.

Mainly experimental techniques and tools in network pharmacology
Technique	Application fields	Advantage	Literatures
HTS/HCS	Massive data acquisition	Homogeneous, multidimensional phenotypic detection, dynamic real-time monitoring, and visualization	[28, 29]
PCR chip	Massive data acquisition	Dual high throughput, strong specificity, high sensitivity, and good repeatability	[30]
SPR	Massive data acquisition	No marks, high-throughput, high-precision, and real-time detection	[31, 32]
BLI	Massive data acquisition	No marks, high-throughput, high-precision, and real-time detection	[31, 32]
Cytoscape	Network visualization	Graphic operation, construct simple network, plugin support for analysis, and easy to use	[37]
GUESS	Network visualization	Graphic operation, command line, and script support for analysis	[38]
Pajek	Network visualization	Graphic operation, building large-scale network	[39]
Network topology information calculation	Network analysis	Classify and sequence the nodes, reflect hidden information	[34]
Random network creation and comparison	Network analysis	Verify reliability of existing network	[35]
Network layer and clustering	Network analysis	Simplify the complexity of network, find out potential information	[36]