Figure 5.

AP-1 signaling is important for miR-155 expression in HTLV-1-positive T cells. (a) DNA-binding activities of proteins to the miR-155 gene promoter in HTLV-1-positive and -negative T-cell lines was assessed by EMSA. The AP-1 oligonucleotide probe containing the AP-1-binding site from miR-155 gene was used. Arrow shows specific DNA-protein complexes. (b) The specificity of AP-1-DNA binding was analyzed in MT-2 cells. Competition assay with cold competitors of wild type probe (WT) or mutated probe (Mut) showed the specificity of the protein-DNA-binding complex. Antibodies against various AP-1 subunits were used for supershift assay. Arrow shows specific complexes of AP-1 with AP-1 probes. Arrowheads show supershifted bands.