Table 3.
Intraday precision and accuracy for QC samples, LLOQ, and ULOQ for alfuzosin in human plasma (EDTA).
| Batch run | HQC | MQC | LQC | LLOQ | ULOQ |
|---|---|---|---|---|---|
| (25 ng/mL) | (2 ng/mL) | (0.150 ng/mL) | (0.050 ng/mL) | (30 ng/mL) | |
| Mean | 25.94 | 2.111 | 0.1543 | 0.04632 | 31.36 |
| SD (n − 1) | 0.7114 | 0.06474 | 0.01073 | 0.001933 | 0.4354 |
| Precision | 2.7 | 3.1 | 7 | 4.2 | 1.4 |
| Accuracy (%) | 104 | 106 | 103 | 92.6 | 105 |
| BIAS (%) | 3.8 | 5.6 | 2.9 | −7.4 | 4.5 |
| n | 6 | 6 | 6 | 6 | 6 |
| Overall mean | 25.88 | 2.069 | 0.1554 | 0.05092 | 30.97 |
| SD (n − 1) | 1.415 | 0.1162 | 0.009395 | 0.004951 | 1.578 |
| Precision | 5.5 | 5.6 | 6 | 9.7 | 8.1 |
| Accuracy (%) | 104 | 103 | 104 | 102 | 103 |
| BIAS (%) | 3.5 | 3.5 | 3.6 | 3.8 | 3.2 |
| n | 18 | 18 | 18 | 18 | 18 |
| Recovery | Internal standards was 68% and analyte was 79% | ||||