Table 1.
Summary of the chromatographic steps of the Bmoo FIBMP-I enzyme from Bothrops moojeni.
| Sample |
Total protein (mg) | Protein recovery (%) | Total activity (units)* | Specific activity** |
|---|---|---|---|---|
| Crude venom | 242.0 | 100 | 35,62 | 147 |
| D4 | 27.38 | 11.31 | 8,310 | 303 |
| D4G2 | 12.06 | 5 (44.041) | 4,544 | 377 |
| Bmoo FIBMP-I | 7 | 2.9 (58.032) | 4,601 | 657 |
*Proteolytic activity was tested with azocasein as substrate.
**One unit of specific activity was defined as units/mg protein.
1Protein recovery related to fraction D4.
2Protein recovery related to fraction D4G2.