Table 1. Primers used for real-time RT-PCR.
Target sequence | Primers | Primer sequence | Band size (bp) |
---|---|---|---|
Rat Pepck BC081900 | Pepck-620-F | 5'-CAGCATGCGGATCATGACACGGAT-3' | 530 |
Pepck-1149-R | 5'-GATTTGTCTTCACTGAGGTGCCCG-3' | ||
Rat G6pase NM_013098 | G6pase-525-F | 5'-CGTCTTGTGGTTGGGATACTG-3' | 164 |
G6pase-688-R | 5'-TCTGGAGGCTGGCATTGTAG-3' | ||
Rat Pgc-1α NM_031347 | Pgc-1α-963-F | 5'-CTCCTCATAAAGCCAACCAAG-3' | 143 |
Pgc-1α-1105-R | 5'-TGTGCGTATAACTCAGATTGC-3' | ||
Rat Trb3 BC091120 | Trb3-187-F | 5′-GCTGCTTCTGCCGATGTTCC-3′ | 209 |
Trb3-395-R | 5′-CAGTTGCCTTGCTCTCGTTCC-3′ | ||
Cyclophilin A (Cph) BC106030 | Cph-96-F | 5'-CTCCTTTGAGCTGTTTGCAG-3' | 325 |
Cph-420-R | 5'-CACCACATGCTTGCCAT-3' |
Abbreviations: G6pase, glucose-6-phophatase; Pepck, phosphoenolpyruvate carboxykinase 1; RT-PCR, reverse transcription PCR.