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. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: Cancer Lett. 2010 Oct 29;300(1):87–95. doi: 10.1016/j.canlet.2010.09.010

Fig. 5.

Fig. 5

Inhibition of proteasomal chymotrypsin-like activity by 1-Cu, 5-Cu and 9-Cu mixtures. (A) The DCIS cells were treated with 0.5, 1.0 and 2.0 μM of the indicated mixtures for 24 h, followed by the measurement of proteasomal CT-like activity. (B) Western blot analysis for the accumulation of ubiquitinated proteins and NOXA induction in the extracts prepared from the DCIS cells for 24 h. (C) The MDA-MB-231 cells were treated with 0.5, 1.0 and 2.0 μM of the indicated mixtures for 24 h, followed by the measurement of proteasomal CT-like activity. (D) Western blot analysis for the accumulation of ubiquitinated proteins and NOXA induction in the extracts prepared from the MDA-MB-231 cells for 24 h. (E) The MDA-MB-231 cells were exposed to a 2.5 μM mixture of 9-Cu and 5-Cu for the indicated times, followed by measurement of proteasomal CT-like activity. (F) Western blot analysis using specific antibodies to ubiquitinated proteins and NOXA in the extracts prepared form the MDA-MB-231 cells for the indicated times.