Table I.

Affinities, dissociation rate constants, R_max and χ² values obtained with SPR for the binding of the C-terminal domain and the full length protein of endolysin PlyL and PlyG to SCWP isolated from different B. anthracis and B. cereus strains (as indicated); analysis using global fitting

SCWPs	ka (1/Ms)	kd (1/s)	KD (M)	Rmax (RU)	χ2	ka (1/Ms)	kd (1/s)	KD (M)	Rmax (RU)	χ2
	PlyL (C-terminal domain)					PlyL (full-length)
Ba34F2	a1.42 × 104	3.23 × 10−2	a1.14 × 10−6	334	9.52	a2.12 × 104	8.88 × 10−2	a7.51 × 10−6	132	1.67
	a3.96 × 10−3	1.51 × 10−3				a7.14 × 10−3	5.67 × 10−3
Ba7702	a3.98 × 104	6.10 × 10−2	a2.66 × 10−6	252	4.99	a4.71 × 104	7.07 × 10−2	a2.50 × 10−6	239	12
	a7.71 × 10−3	4.98 × 10−3				a7.64 × 10−3	5.10 × 10−3
BcG9241	a9.95 × 103	6.49 × 10−2	a1.68 × 10−5	98	0.65	a1.58 × 104	3.74 × 10−1	a2.63 × 10−5	198	1.76
	a4.27 × 10−3	6.76 × 10−3				a3.35 × 10−2	3.92 × 10−3
Bc14579	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
Bc10987	NB	NB	NB	NB	NB	NB	NB	NB	NB	NB
	PlyG (C-terminal domain)					PlyG (full length)
Ba34F2	b3.69 × 103	c3.01 × 10−3	d0.81 × 10−6	ND	ND	b5.22 × 103	c4.25 × 10−3	d0.81 × 10−6	ND	ND
Ba7702	b6.62 × 103	c4.16 × 10−3	d0.62 × 10−6	ND	ND	b3.28 × 103	c4.60 × 10−3	d1.40 × 10−6	ND	ND
BcG9241	b1.1 × 104	c3.82 × 10−3	d0.34 × 10−6	ND	ND	b1.17 × 103	c2.9 × 10−3	d2.47 × 10−6	ND	ND
Bc14579	NB	NB	NB	NB	NB	ND	ND	ND	ND	ND
Bc10987	NB	NB	NB	NB	NB	ND	ND	ND	ND	ND

NB, no binding; ND, not determined.

^aAssociation and dissociation phase were fitted to a two-state reaction model.

^bAssociation phases were calculated from the equation k_obs= k_aC+k_d.

^ck_d was calculated using the 1:1 Langmuir dissociation model.

^dK_D was calculated from k_a and k_d values.