Table II.
Anti-laminin Ig from LamH Ig Tg+ galectin-1−/− subjects with or without immunization
| Subject(s) | Serum (OD405) | B cell supernatant (OD405) |
|---|---|---|
| Laminin immunization | ||
| Mouse 1 | 0.000 | 0.000 |
| Mouse 2 | 0.002 | 0.249 |
| Mouse 3 | 0.008 | 0.048 |
| Unimmunized | ||
| Mean OD ± SD (n = 4–6) | 0.016 ± 0.019 | 0.019 ± 0.011 |
Shown is OD405 for Ig binding on laminin-coated wells minus binding on diluent-coated wells. Sera were assayed at 1:20 dilution, supernatants from LPS-stimulated B cells were assayed undiluted for n = 3 LamH Ig Tg+ galectin-1−/− galectin-3+/− mice. Summary values for unimmunized LamH Ig Tg+ Gal-1−/− mice are shown for reference.