Table I.
Results of combined application of ethanol (EH) and cytochalasin B (CB).
| Group | Oocyte (MII) n | Cleavage n (%) | Overspeed n (%) | Blastulation n (%) | Blastocyst hatching n (%) |
|---|---|---|---|---|---|
| 1 | 462 | 441 (95.5) | 29 (6.58) | 233 (43.3) | 38 (8.62) |
| 2 | 431 | 407 (94.4) | 33 (8.11) | 126 (31.0) | 10 (2.46) |
| 3 | 437 | 397 (90.8) | 69 (17.4) | 52 (13.1) | 1 (0.25) |
| 4 | 385 | 281 (73.0) | 12 (4.27) | 22 (7.83) | 0 (0.00) |
| 5 | 397 | 70 (17.6) | 3 (2.86) | 3 (4.28) | 0 (0.00) |
| 6 | 358 | 44 (12.3) | 1 (2.27) | 1 (2.27) | 0 (0.00) |
Group 1, activated with EH plus CB for 5 min; group 2, activated with EH plus CB for 7 min; group 3, activated with EH plus CB for 10 min; group 4, activated with EH for 7 min, then with CB for 7 min; group 5, activated with CB for 7 min; group 6, treated with culture liquid (control). The final concentration of EH was 7% and of CB was 5 μg/ml. Overspeed, cleavage rate is faster than that of the normal zygote.