Table II.
Results of combined application of strontium chloride (SrCl2) and cytochalasin B (CB).
| Group | Oocyte (MII) n | Cleavage n (%) | Overspeed n (%) | Blastulation n (%) | Blastocyst hatching n (%) |
|---|---|---|---|---|---|
| 1 | 391 | 305 (78.0) | 26 (8.52) | 84 (27.6) | 11 (3.61) |
| 2 | 404 | 334 (82.7) | 51 (15.3) | 47 (14.1) | 4 (1.19) |
| 3 | 370 | 331 (89.7) | 71 (21.5) | 24 (7.25) | 0 (0.00) |
| 4 | 389 | 277 (71.2) | 18 (6.50) | 7 (2.53) | 1 (0.36) |
| 5 | 316 | 52 (16.5) | 3 (5.76) | 1 (1.92) | 0 (0.00) |
| 6 | 338 | 37 (10.9) | 2 (5.40) | 0 (0.00) | 0 (0.00) |
Group 1, activated with SrCl2 plus CB for 30 min; group 2, activated with SrCl2 plus CB for 1 h; group 3, activated with SrCl2 plus CB for 2 h; group 4, activated with SrCl2 for 1 h, then with CB for 7 min; group 5, activated with CB for 7 min; group 6, treated with culture liquid (control). The final concentration of SrCl2 was l0 mmol/l and of CB was 5 μg/ml. Overspeed, cleavage rate is faster than that of the normal zygote.