Table IV.
Comparison of the related data of three chemical combination methods.
| Group | Cleavage number (%) | Blastulation number (%) | Blastocyst hatching number (%) |
|---|---|---|---|
| 1 (EH + CB) | 95.5 | 43.3 | 8.62 |
| 1 (SrCl2 + CB) | 78.0 | 27.6 | 3.61 |
| 1 (Ino + CB) | 65.9 | 15.8 | 1.66 |
| CB (EH + CB) | 17.6 | 4.28 | 0.00 |
| CB (SrCl2 + CB) | 16.5 | 1.92 | 0.00 |
| CB (Ino + CB) | 18.0 | 0.00 | 0.00 |
| Control (EH + CB) | 12.3 | 2.27 | 0.00 |
| Control (SrCl2 + CB) | 10.9 | 0.00 | 0.00 |
| Control (Ino + CB) | 11.4 | 0.00 | 0.00 |
| DMSO | 11.5 | 0.00 | 0.00 |
Group 1 (EH + CB), activated with EH plus CB for 5 min; group 1 (SrCl2 + CB), activated with SrCl2 plus CB for 30 min; group 1 (Ino + CB), activated with ionomycin plus CB for 3 min. Groups CB (EH + CB) and CB (SrCl2 + CB), activated with CB alone for 7 min; Group CB (Ino + CB), activated with CB alone for 5 min; Groups Control (EH + CB) and Control (SrCl2 + CB), cultured in the absence of activator for 7 min; Group CB (Ino + CB), cultured in the absence of activator for 5 min; Group DMSO, cultured with DMSO for 5 min. CB, cytochalasin B; EH, ethanol; SrCl2, strontium chloride; Ino, ionomycin calcium; DMSO, dimethyl sulfoxide.