Figure 1. In vitro phosphorylation of Rh2b by parasite lysate.

(A) Primary sequence of the CPD of AMA1 and Rh2b. Amino acids predicted to be phosphorylated are shown in large font. Partial sequence of the transmembrane domain is depicted in small font. (B) In vitro radioactive [γ-³²P]ATP phosphorylation assays of recombinant GST–AMA1 and GST–Rh2b fusion proteins in the presence (+) or absence (−) of 10 μM cAMP using schizont parasite lysate. Purified GST was used as a control. The loading of recombinant proteins is shown in the Coomassie Blue-stained SDS/PAGE. (C) In vitro radioactive [γ-³²P]ATP phosphorylation assays of recombinant GST–AMA1, GST–Rh2b and GST using recombinant MmPKAc. The molecular mass is shown in kDa on the left-hand side of the blots.