Gel electrophoresis to test pDNA retention in copolymer/pDNA polyplexes at various N/P ratios. (A) Without 10% FBS and (B) with 10% FBS.
Note: Naked pDNA was used as a reference, and numerals of each graph indicate an N/P ratio.
Abbreviations: pDNA, plasmid deoxyribonucleic acid; N/P, nitrogen/phosphate; PF127-pDMAEMA, pluronic F127-poly (dimethylaminoethyl methacrylate); PF127-p(DMAEMA-tBA), pluronic F127-poly (dimethylaminoethyl methacrylate-tert-butyl acrylate); PF127-p(DMAEMA-AA), pluronic F127-poly (dimethylaminoethyl methacrylate-acrylic acid); DNA, deoxyribonucleic acid; FBS, fetal bovine serum.