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. 2013 May 27;8:2011–2027. doi: 10.2147/IJN.S44222

Figure 9.

Figure 9

CLSM images of 293T cells exposed to copolymer/Cy5-labeled pGL3 polyplexes at N/P = 9 for 4 hours of incubation.

Note: Blue: nuclei (Hoechst 33342), green: endolysosome (Lysotracker), and red: Cy5-labeled pGL3 plasmid DNA.

Abbreviations: CLSM, confocal laser scanning microscope; N/P, nitrogen/phosphate; pDNA, plasmid deoxyribonucleic acid; DIC, differential interference contrast; PF127-pDMAEMA, pluronic F127-poly (dimethylaminoethyl methacrylate); PF127-p(DMAEMA-tBA), pluronic F127-poly (dimethylaminoethyl methacrylate-tert-butyl acrylate); PF127-p(DMAEMA-AA), pluronic F127-poly (dimethylaminoethyl methacrylate-acrylic acid).