Table 2.
Hydrodynamic diameters of copolymer/pDNA polyplexes in DD water with and without 10% FBS after left to stand for 10 minutes or 4 hours
| Polyplex (N/P = 9) | Dh (nm) at 10 minutes | PDI | Dh (nm) at 4 hours | PDI | Dh (nm) at 4 hours with FBS | PDI |
|---|---|---|---|---|---|---|
| PF127-pDMAEMA/pDNA | 102 ± 11 | 0.28 ± 0.02 | 103 ± 10 | 0.27 ± 0.02 | 225 ± 30 | 0.35 ± 0.11 |
| PF127-p(DMAEMA-tBA)/pDNA | 97 ± 8 | 0.30 ± 0.03 | 108 ± 9 | 0.26 ± 0.03 | 142 ± 15 | 0.33 ± 0.09 |
| PF127-p(DMAEMA-AA)/pDNA | 82 ± 7 | 0.26 ± 0.02 | 92 ± 5 | 0.25 ± 0.03 | 284 ± 47 | 0.41 ± 0.12 |
Note: n = 3.
Abbreviations: pDNA, plasmid deoxyribonucleic acid; DD water, double-deionized water; FBS, fetal bovine serum; N/P, nitrogen/phosphate; Dh, hydrodynamic diameters; PDI, polydispersity index; PF127-pDMAEMA, pluronic F127-poly (dimethylaminoethyl methacrylate); PF127-p(DMAEMA-tBA), pluronic F127-poly (dimethylaminoethyl methacrylate-tert-butyl acrylate); PF127-p(DMAEMA-AA), pluronic F127-poly (dimethylaminoethyl methacrylate-acrylic acid).