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. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: Nat Immunol. 2011 Sep 4;12(10):959–965. doi: 10.1038/ni.2091

Figure 7.

Figure 7

The Walker motifs in DDX41 are essential for sensing DNA. (a) Full-length DDX41 (X41) and serial deletion mutants of DDX41 lacking the HELICc domain (X41-e), both the Walker A motif and the HELICc domain (X41-dA) or both the Walker B motif and the HELICc domain (X41-dM). (b) Immunoblot analysis of immunoprecipitation assays of purified HA-tagged DDX41 (as in a) incubated with biotinylated poly(dG:dC) followed by addition of avidin beads (top), or with Myc-tagged STING, followed by the addition of anti-Myc beads (bottom), probed with anti-HA. Input, 10% of the purified HA-tagged DDX41. (c) Activation of the Ifnb promoter in L929 cells transfected with the IFN-β luciferase reporter (100 ng) plus increasing concentrations (20, 100 or 200 ng; wedges) of expression vectors for DDX41 (as in a); a renilla luciferase reporter (2 ng) was transfected simultaneously as an internal control. Results are presented relative to those of cells transfected with empty vector alone (Vector). Data are representative of three independent experiments (mean and s.d. in c).