Table 1.
Kinetic constants of the light-driven system compared to the compared to the NADPH-dependent system
| Substrate | PSI-Fdx-CYP124
|
NADPH-FdR-Fdx-CYP124
|
||
|---|---|---|---|---|
| KM (μM) | kcat (min−1) | KM (μM) | kcat (min−1) | |
| Farnesol | 3.4 ± 1.1 | 3.1 ± 0.2 | 36 ± 3a | 15.5 ± 2.8b |
| Cholest-4-en-3-one | 8.5 ± 2.2 | 2.8 ± 0.2 | 21 ± 2.1a | 11.7 ± 1.3b |
The farnesol and cholest-4-en-3-one concentrations used were 0–50 and 0–75 μM, respectively. Otherwise, the experimental setup for determining the kinetic constants was identical to the procedure described in the materials and methods section
a
Values from Johnston et al. (2010)
b
Values from Johnston et al. (2009)