Fig. 2.

Glyoxal inhibits MTT reduction by endothelial cells. BPAECs (90% confluent), grown in 17.5-mm dishes (24-well culture plate) were treated with MEM alone or MEM-containing glyoxal (1, 5, and 10 mM) for a 4 h, b 6 h, or c 12 h and incubated at 37°C in a humidified environment of 5% CO₂–95% air. At the end of incubation, MTT reduction (as an index of cytotoxicity) was determined spectrophotometrically as described under Materials and methods section. Data represent means ± SD of three independent experiments. * Significantly different at P < 0.05 as compared to the cells treated with MEM alone