Fig. 7.

Aminoguanidine (AG) protects against glyoxal-induced alterations in cell morphology. BPAECs (90% confluent), grown in 35-mm dishes (6-well culture plate) were treated with MEM alone or MEM-containing a glyoxal 1 mM for 6, 12, and 24 h. Cells were then examined after pre-treatment with MEM alone or MEM-containing b AG HCl or c AG nitrate (1, 5, and 10 mM) for 1 h then treated with MEM alone, MEM-containing AG HCl or AG nitrate (1, 5, and 10 mM), MEM-containing glyoxal (1 mM), or MEM-containing AG HCl or AG nitrate (1, 5, and 10 mM) + glyoxal (1 mM) for 12 h at 37°C in a humidified environment of 5% CO₂–95% air. At the end of the incubation period, cells were examined under light microscope at a magnification of 20× as described under Materials and methods section. Each micrograph is a representative picture obtained from three independent experiments conducted under identical conditions