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. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: J Immunol. 2010 Jun 23;185(3):1822–1835. doi: 10.4049/jimmunol.0903398

FIGURE 2.

FIGURE 2

Induction of phenotypic changes in human peripheral monocytes by sRAGE. CD14+ monocyte (5 × 106) cells were cultured in a six-well plate with X-VIVO 15 medium supplemented with 10 μg/ml polymyxin B sRAGE (0.25–10 μg/ml) in a 37°C incubator. Cells cultured with either M-CSF or GM-CSF served as positive controls. Pictures were taken after 1 d in culture using an Olympus inverted microscope (Olympus) (original magnification × 100). The inset in each picture is an enlarged section of the original image. Shown are representative pictures obtained from six independent donors performed in duplicate.