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. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: J Immunol. 2010 Jun 23;185(3):1822–1835. doi: 10.4049/jimmunol.0903398

FIGURE 5.

FIGURE 5

Activation of downstream signaling in human monocytes and monocytes derived macrophages by sRAGE. Protein lysates were generated and immunoblotted for phosphorylation of Akt and Erk from monocytes (A) or MDMs (B) stimulated with 5 μg/ml sRAGE for indicated times. Equal loading was confirmed by β-actin Ab. Shown are representative data from monocytes or macrophages obtained from three independent donors. Phosphorylation levels were quantified by comparing band densitometry of the upper panels and normalizing these values to the β-actin signals in the respective lanes for monocytes (C) and MDMs (D). Shown is the average fold increase ± SEM over NS controls from three independent donors.