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The iPOND procedure consists of pulsing cells with EdU to label nascent DNA in vivo, formaldehyde crosslinking protein-DNA complexes, covalently tagging EdU-labeled DNA with biotin using click chemistry, lysing and sonicating cells, purifying the solubilized protein-DNA complexes, and eluting bound proteins for analysis by SDS-PAGE and immunoblotting or MS. Numbering corresponds to the procedure step number
