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Click reaction cocktails for a sample with 1 × 10 cells. Adjust volumes proportionally for actual cell number.
| Reagent | [stock] | [final] | Control reaction volume (ml) |
Experimental reaction volume (ml) |
|---|---|---|---|---|
| 1×PBS | 4.35 | 4.35 | ||
| DMSO | 0.05 | |||
| Biotin-azide | 1mM | 10μM | 0.05 | |
| Sodium ascorbate | 100mM | 10mM | 0.5 | 0.5 |
| CuSO4 | 100mM | 2mM | 0.1 | 0.1 |
| Total Volume | 5.0 | 5.0 |
