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. 2013 May 28;8:17. doi: 10.1186/1750-1326-8-17

Figure 4.

Figure 4

αS aggregate formation in neurons and astrocytes. (A) αS pSer129 positive aggregates (green) induced by treatment with extracellular αS fibrils in cultures with rAAV2/1 human αS expression can occur in neurons as shown by double immunofluorescence with the neuronal marker MAP2B (red). (B) αS pSer129 positive aggregates (green) induced by treatment with extracellular αS fibrils in cultures with rAAV2/1 human αS expression can occur in astrocytes as shown by double immunofluorescence with the astrocyte marker GFAP (red). Cultures were counter stained with DAPI (blue). Bar scale = 25 μm.