Figure 5.

αS inclusions in the mixed neuronal-glial cultures co-localize with ubiquitin. (A) In cultures treated with rAAV2-1 human wild-type αS, pSer129 (red) neuritic background staining does not co-localize with endogenous ubiquitin (green). (B) In cultures treated with rAAV2/1 human wild-type αS and exogenous human wild-type αS fibrils, pSer129 (red) hyperphosphorylated αS inclusions in these cultures are ubiquitinated (green). This ubiquitin staining profile (green) co-localizes with the neuronal marker MAP2B (red, C) and the astrocyte marker GFAP (red, D). Cultures were counter stained with DAPI (blue). Bar scale = 25 μm.