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αS inclusions induced in mixed neuronal-glial cultures contain Thioflavin- S positive structures. (A) In cultures treated with rAAV2/1 human wild-type αS and human wild-type αS fibrils, pSer129 (red) hyperphosphorylated αS inclusions were also ThS positive (green). This ThS staining profile (green) co-localizes with the neuronal marker MAP2B (red, B) and the astrocyte marker GFAP (red, C). Cultures were counter stained with DAPI (blue). Bar scale = 50 μm.
