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. 2013 Jan 30;38(5):729–742. doi: 10.1038/npp.2012.246

Figure 3.

Figure 3

GLYX-13 but not ketamine produces an antidepressant-like effect in the rat Porsolt test when injected into the medial prefrontal cortex. Mean (±SEM) time (sec) spent immobile in the Porsolt test in 2–3-month-old male rats implanted with (a) medial prefrontal or motor cortex (dorsal control) cannulae and injected with GLYX-13 ( 0.1, 1, 10 μg/side) or sterile saline vehicle (0.5 μl/1 min) and tested 1 h post-dosing or rats given MPFC injections of ketamine (0.1, 1, 10 μg), GLYX-13 (1 μg), or saline and tested (b) 20 min and (c) 24 h post dosing. Animals received a 15-min training swim session 1 day before dosing. (d) Mean (± SEM) line crosses in the open field 20 min; following MPFC infusion of GLYX-13 (1 μg), ketamine (0.1 μg) or sterile saline vehicle. Given that 0.1 μg dose of ketamine increased locomotor activity, the Porsolt data for that dose were not included in the analysis given that increasing locomotor activity produces a false-positive antidepressant-like response. (e) a representative H&E-stained section depicting MPFC cannulae placement, arrow indicates injection site. n=5–10 per group. *P<0.05, Fisher's PLSD vs vehicle.