. 2013 May 29;13:117. doi: 10.1186/1472-6882-13-117

Table 3.

Identification by nano HPLC-ESI-MS/MS of S. mutans membrane proteins differentially expressed when treated with 2x LMM fraction of mushroom extract

Protein name	Spot no.	Fold of variation	NCBI acc. #	Gene name	Theor. Mr. (Da)	Theor. pI	No. of peptides identified	Mascot score^a)	Sequence coverage^b)(%)	Molecular function
*MEMBRANE TRANSPORT*
F0F1 ATP synthase subunit delta	2202	- 2.27	gi\|24379920	atpH	20436	5.22	9	528	51	ATPase activity
Putative ABC transporter, ATP-binding protein	5303	- 2.08	gi\|24380277	psaA	26297	5.58	12	694	57	ATPase activity
Putative oligopeptide ABC transporter, ATP-binding protein	5601	- 2.38	gi\|24378766	oppD	39147	5.47	15	680	56	ATPase activity
Putative sugar ABC transporter, ATP-binding protein	5804	- 7.32	gi\|24379552	SMU_1120	55575	5.78	26	1197	62	ATPase activity
Putative amino acid ABC transporter, ATP-binding protein	6406	- 2.56	gi\|24378949	SMU_461	27674	5.83	19	847	75	ATPase activity
Putative amino acid ABC transporter, ATP-binding protein	7303	- 9.09	gi\|24379380	SMU_936	28340	5.90	2	118	10	ATPase activity
Putative phosphate ABC transporter, ATP-binding protein	7304	- 2.17	gi\|24379566	pstB	30276	5.90	3	136	12	ATP binding
Multiple sugar-binding ABC transporter, ATP-binding protein	7702	- 2.94	gi\|24379336	msmK	41938	5.92	14	579	39	ATPase activity
*SUGAR BIOSYNTHESIS AND METABOLISM*
Putative glucose-1-phosphate thymidyltransferase	2402	- 2.50	gi\|24379858	rmlA	32275	4.90	16	917	53	Tranferase activity
Pyruvate kinase	4101	+ 2.76	gi\|24379618	pykF	54333	5.09	2	70	5	Kinase activity
Glyceraldehyde-3-phosphate dehydrogenase	4601	+ 2.28	gi\|24378857	gapC	36046	5.71	17	725	44	Oxidoreductase activity
Galactose 6-P isomerase	6102	+ 3.01	gi\|153674	lacA	19025	5.80	2	84	15	Isomerase activity
Catabolite control protein A	8501	- 6.14	gi\|2155300	ccpA	36590	7.08	3	124	9	DNA binding
*PROTEIN BIOSYNTHESIS AND FOLDING*
60 kDa chaperonin	5102	+ 2.19	gi\|13898679	cpn60	19827	4.53	2	87	14	Protein binding
Phenylalanyl-tRNA synthetase subunit alpha	6603	- 7.83	gi\|24379902	pheS	39271	5.87	14	420	34	Nucleotide binding
30S ribosomal protein S8	9103	+ 2.24	gi\|24380355	rpsH	14684	9.10	7	411	64	RNA binding
*CELL CYCLE AND DIVISION*
Putative Hit-like protein	2102	+ 2.07	gi\|24378905	SMU_412c	15560	5.13	3	139	30	Unknown
Hypothetical protein SMU.471	4002	- 2.04	gi\|24378958	SMU471	12972	5.37	5	255	42	Methylase activity
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1	5702	+ 2.30	gi\|24379914	murA1	45588	5.65	10	422	30	Tranferase activity
*AMINOACIDS BIOSYNTHESIS AND METABOLISM*
Uridylate kinase	3302	+ 4.08	gi\|24380005	pyrH	26315	5.48	9	460	52	Kinase activity
Phospho-2-dehydro-3-deoxyheptonate aldolase	4505	+ 2.60	gi\|24380198	aroG	39072	6.02	8	338	29	Lyase activity
Shikimate 5-dehydrogenase	5401	+ 2.36	gi\|24379237	aroE	31791	6.19	2	106	11	Oxidoreductase activity
Putative D-3-phosphoglycerate dehydrogenase	5604	- 3.33	gi\|24380031	serA	42771	5.56	14	815	51	Oxidoreductase activity
*MISCELLANEOUS*
Putative dihydrolipoamide dehydrogenase	3603	+ 4.23	gi\|24378648	adhD	61708	4.88	4	270	10	Oxidoreductase activity
Orotate phosphoribosyltransferase	4201	+ 2.38	gi\|24379645	pyrE	22802	5.37	7	381	45	Glycosyltransferase activity
Putative exodeoxyribonuclease III	6403	- 2.56	gi\|24380027	smnA	31393	5.72	11	558	44	DNA binding
(3R)-hydroxymyristoyl-ACP dehydratase	7403	- 2.08	gi\|24380109	fabZ	15361	8.83	3	150	21	Lyase activity
Not identified	5301	+ 4.52
Not identified	8205	+ 2.59
Not identified	8401	- 4.35
Not identified	8506	+ 3.30

^a) Score is –10⁶log(p), where p is the probability that the observed match is a random event, based on the NCBI database using the MASCOT searching program as MS/MS data.

^b) Amino acid sequence coverage for the identified protein.