. 2013 May 29;13:117. doi: 10.1186/1472-6882-13-117

Table 4.

Identification by nano HPLC-ESI-MS/MS of S. mutans cytosol proteins differentially expressed when treated with 2x LMM fraction of mushroom extract

Protein name	Spot no.	Fold of variation	NCBI acc. #	Gene name	Theor. Mr. (Da)	Theor. pI	No. of peptides identified	Mascot score^a)	Sequence coverage^b)(%)	Molecular function
*STRESS RESPONSE*
60 kDa chaperonin	1605	+ 2.17	gi\|21666296	groEL	56428	4.68	39	2392	78	Protein binding
Molecular chaperone dnaK	1706	+ 2.29	gi\|24378606	dnaK	65246	4.58	28	1265	51	Protein binding
10 kDa chaperonin	3001	+ 3.25	gi\|21666295	groES	10064	4.93	4	273	32	Protein binding
Superoxide dismutase	4108	+ 2.60	gi\|24379102	sodA	22611	4.99	9	521	56	Ion binding
Glutathione reductase	6607	+ 3.89	gi\|4587160	gshR	48955	5.40	3	143	7	Oxidoreductase activity
*SUGAR BIOSYNTHESIS AND METABOLISM*
Glucose-1-phosphate adenylyltransferase	2501	- 3.57	gi\|92081399	glgC	41906	4.72	13	680	38	Transferase activity
Phosphopyruvate hydratase	2806	- 2.56	gi\|24379669	eno	46829	4.67	14	771	40	Lyase activity
Putative phosphoglucomutase	3703	- 12.50	gi\|24379514	pgm	63056	4.88	33	1783	69	Transferase activity
Tagatose 1,6-diphosphate aldolase 1	4303	- 2.17	gi\|153676	lacD1	36468	4.96	11	462	31	Lyase activity
Putative N-acetylglucosamine-6-phosphate isomerase	6205	+ 2.43	gi\|24379109	SmuNN2025_1351	25457	5.33	7	330	37	Deaminase activity
Fructose bi-phosphate aldolase	8001	+ 5.24	gi\|4322370	fba	7447	8.85	3	115	30	Lyase activity
*PROTEIN BIOSYNTHESIS AND FOLDING*
Elongation factor Tu	1603	- 2.44	gi\|24379182	tuf	43891	4.84	25	1257	72	GTPase activity
Trigger factor	1701	+ 2.06	gi\|24378614	tig	47457	4.46	20	918	51	Isomerase activity
50S ribosomal protein L4	3101	+ 2.18	gi\|254998187	SmuNN2025_1762	22215	9.76	6	313	39	RNA binding
Elongation factor Ts	3503	- 2.38	gi\|24380373	tsf	37695	4.91	22	1218	74	GTP binding
*CELL REDOX HOMEOSTASIS*
Thiol peroxidase	0007	+ 4.33	gi\|254997527	tpx	17541	4.56	8	450	34	Oxidoreductase activity
Alkyl hydroperoxide reductase	1107	+ 12.60	gi\|24379223	ahpC	20465	4.57	6	284	34	Oxidoreductase activity
Putative bacterocin transport accessory protein, Bta	5002	+ 2.71	gi\|24380154	SMU_1788c	12601	5.21	2	85	18	Oxidoreductase activity
Putative glutathione S-transferase	8202	+ 5.64	gi\|24379712	SMU_1296	29973	5.74	5	243	16	Transferase activity
Not identified	8007	- 3.57

^a) Score is –10⁶log(p), where p is the probability that the observed match is a random event, based on the NCBI database using the MASCOT searching program as MS/MS data.

^b) Amino acid sequence coverage for the identified protein.