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. 2013 Jun 4;8(6):e65222. doi: 10.1371/journal.pone.0065222

Figure 1. Schematic representation of the adenoviral genomes used in this study.

Figure 1

Ad-dl309 encodes both E1a and E1b genes including their respective Ad promoters (pE1a and pE1b, respectively). The virus has a deletion in the E3 region spanning 30005–30750 bp [81]. Ad-dl309ΔVA and Ad-dl1520 have additional deletions in the VA-RNA genes (10667–10702 bp as well as 10929–10943 bp) or in the E1b-55K gene, respectively. AdΔE1b, AdΔE1bΔVA and AdControl (ΔE1aΔE1b) were constructed using the AdEasy-1 system and the pAdTrack shuttle plasmid. The pAd-Easy-1 plasmid has a larger deletion in the E3 region spanning 28130–30820 bp. The pAd-Track plasmid has E1 sequences spanning 480–3533 bp replaced with the EGFP gene under the control of the human cytomegalovirus immediate early (hCMV) promoter [53]. The E1a gene under the control of the murine cytomegalovirus immediate early (mCMV) promoter was introduced back into the genomes of AdΔE1b and AdΔE1bΔVA. Additionally, AdΔE1bΔVA has the same VA-RNA deletions as Ad-dl309ΔVA.