Figure 3. 2′AP increases the replication of adenoviruses with an E1b deletion or with both E1b and VA-RNA deletions.

(A) Cells were infected in duplicate with Ad-dl309 and AdΔE1b at an MOI of 1 PFU/cell 1 hour prior to treatment with medium containing no drug or 2.5 mM 2′AP. Cells and media were harvested at 1 hr (day 0) as well as 1, 2, 3 and 4 days post-infection. Virus yields were determined using plaques assays on HEK293 cells. (B) Cells were infected with AdΔE1b and AdΔE1bΔVA at an MOI of 1 GFU/cell 1 hour prior to addition of medium containing no drug or 2.5 mM 2′AP. Cells and media were harvested at 1 hr (day 0) as well as 1, 2, 3 and 4 days post-infection. Virus yields were determined by titration in Hep3B cells. Error bars correspond to +/−SD.