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. 2013 Jun 4;8(6):e65222. doi: 10.1371/journal.pone.0065222

Figure 7. p53 expression and activity were not inhibited by 2′AP treatment of infected cells.

Figure 7

(A) HepG2 and Hep3B cells were infected with Ad-dl309, Ad-dl1520 or AdΔE1b at MOI of 100 VP/cell and treated with no drug or 2.5 mM 2′AP for 2 days. p53 and E1a levels were detected by western blot analysis. (B) HepG2 and Hep3B cells were transfected with a p53-responsive firefly luciferase expression vector. The next day, transfected cells were mock infected or infected with Ad-dl309, Ad-dl1520 or AdΔE1b at MOI of 100 VP/cell and treated with no drug or 2.5 mM 2′AP. Luciferase expression was measured 2 days post-infection. Both HepG2 and Hep3B have high transfection efficiencies. Error bars correspond to +/−SD of triplicates.