Figure 8. 2′AP rescued the C454S/C456S E1b-55K mutation in HepG2 cells.

(A) Parental HepG2, HepG2-E1b-WT and HepG2-E1b-Mut were infected with Ad-dl309, Ad-dl1520 or AdΔE1b at MOI of 1 PFU/cell for 1 hour prior to treatment with medium containing no drug or 2.5 mM 2′AP. Infected cells and media were harvested 4 days post-infection and virus yields were determine by plaque assays on HEK293 cells. (B) Cells were infected with AdΔE1b at MOI of 1 GFU/cell for 1 hour prior to treatment with medium containing no drug or 2.5 mM 2′AP. Lysates from infected cells were harvested at 1 hr (day 0) as well as 1, 2, 3 and 4 days post-infection. Virus yields were determined by titration in Hep3B cells as described in the materials and methods. Error bars correspond to +/−SD of quadruplicates (NS – Not Significant; ****p<0.0001, **p<0.01, one-way ANOVA).