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A673/TR/LOX-PP cells (clone 2) were incubated in the absence or in the presence of doxycycline (DOX, 1 µg/ml, 48 hours) and the levels of activates P-AKT (Ser 473) and P-ERK (Tyr 204) determined by western-blot. The same blot was stripped and successively incubated with anti-AKT, anti-ERK and anti-α-tubulin as controls for loading and transferring.
