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. 2013 Jun 4;8(6):e64365. doi: 10.1371/journal.pone.0064365

Figure 2. Immunogenicity of the wild type and enhanced POTE 252 epitopes in AAD mice.

Figure 2

(A) Mice were immunized with 50 nmol of POTE 252 in 100 µl of emulsion, and targets were pulsed with 1.0 µM POTE252. Spots were counted by an ELISPOT reader (AID ELISPOT reader system). Figures show numbers of spots per million cells. (B) CTL cross-reactivity on each peptide. Two weeks after the second boost, pooled spleen cells from three mice were restimulated with irradiated splenocytes pulsed with 1.0 µM each peptide for 7 days. Then a 4 hour 51Cr release assay was performed. (C) HLA-A2/peptide tetramer staining and (D) TCR repertoire determination for POTE 252- and POTE 252-9V-induced CTLs in HHD-2 mice.