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. 2013 Jun 4;8(6):e65269. doi: 10.1371/journal.pone.0065269

Figure 1. Western blot analysis of unknown testis-enriched protein in mouse and swamp eel.

Figure 1

a. Western blot analysis using anti-NF-kB1 antibody in HeLa cells and the swamp eels showed two bands: 50kD (P50) and 105kD (P105) (stars denote the bands) in HeLa cells (two repeat samples), while only a dominant 70 KD band in testis of the swamp eel. β-actin protein was used as an internal control. Molecular weight sizes for proteins were shown on the right. b. Immunoprecipitation using anti-NF-kB1 antibody enriched the unknown testis-enriched protein in the swamp eel and mouse. Testis samples showed more obvious band of 70 kD in the swamp eel and mouse. Stars denote the band.