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. 2013 Jun 4;8(6):e65269. doi: 10.1371/journal.pone.0065269

Table 1. The primers for sequencing.

Primer name Primer sequences (5′-3′) PCR
Hspa1a
Hspa1a(F1) ATGTCTGCAGCTAAAGGTGT 94°C, 30 s; 55°C, 30 s; 72°C, 2 min; 35cycles
Hspa1a(R1) TCAATCTACCTCCTCAATAG
Hspa1b
Hspa1b(F1) ATGTCCTCAGCTAAAGGAAT 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa1b(R1) TTAGTCCACTTCCTCAATAG
Hspa4a
Hspa4a(F1) ATGGCAGTTGTCGGATTTGA 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa4a(R1) TTAATCAAGGTCCATGTCAG
Hspa4b
Hspa4b(F1) ATGTCAGTGGTGGGATTTGA 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa4b(R1) TTAGTCAATGTTCATTTCAG
Hspa4L
Hspa4L(F1) ATGTCAGTGGTAGGCATTGA 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa4L(R1) CTCCATCTCTTTCGTGCCAG
Hspa5
Hspa5(F1) ATGAAGCTGTTATGGGTTGT 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa5(R1) CTACAACTCATCCTTCTCAT
Hspa8a1
Hspa8a1(F1) ATGTCTAAAGGACCAGCAG 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa8a1(R1) TTAGTCGACCTCTTCAATGG
Hspa8a2
Hspa8a2(F1) ATGTCAGTGGTAGGCATTGA 94°C, 30 s; 57°C, 30 s; 72°C, 2 min; 35cycles
Hspa8a2(R1) TCAGTCGACTTCCTCGATGG
Hspa8b1
Hspa8b1(F1) ATGTCCAAGGGACCAGCAGT 94°C, 30 s; 61°C, 30 s; 72°C, 2 min; 35cycles
Hspa8b1(R1) TTAGTCAACCTCCTCAATGG
Hspa8b2
Hspa8b2(F1) ATGTCTAAAGGACCAGCAGT 94°C, 30 s; 57°C, 30 s; 72°C, 2 min; 35cycles
Hspa8b2(R1) CTGGTATAGCTTGGAGATGA
Hspa9
Hspa9(F1) GGTTTCCAGCCAGATGTTCT 94°C, 30 s; 57°C, 30 s; 72°C, 2 min; 35cycles
Hspa9(R1) TTAATCGTCATGACATATTG
Hspa12a
Hspa12a(F1) ATGGCTAACCCTTCTCCAGC 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa12a(R1) TTAATGGCTCAGGAAGTCAA
Hspa12b
Hspa12b(F1) GACAGTCCATCAGCCCCGTC 94°C, 30 s; 59°C, 30 s; 72°C, 2 min; 35cycles
Hspa12b(R1) TCAGTTAGACAGGAAGTCTA
Hspa14
Hspa14(F1) ATGGCTGCGATTGGAGTCCA 94°C, 30 s; 61°C, 30 s; 72°C, 2 min; 35cycles
Hspa14(R1) TTATGAAGCAGCCGCTATGG