Table 2. Primers for semi-quantitative RT-PCR analysis.

Primer name	Primer sequences(5′–3′)	PCR
Hspa1a
Hspa1a(F2)	TCCCAGCGACAGGCGACTAA	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa1a(R2)	CTGGCTGATGTCCTTCTTGTGC
Hspa1b
Hspa1b(F2)	ACGAAATTGTCCTGGTTGGC	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa1b(R2)	GGTATTGCGTTTGATTAGTGGTGT
Hspa4a
Hspa4a(F2)	CAGTTGAAATAGTGGGTGGAG	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa4a(R2)	AGGAGATGGGATAAGGAA
Hspa4b
Hspa4b(F2)	CAGTGACTTTAGCGTATGG	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa4b(R2)	CTCGCAGAAGTGGTTGA
Hspa4L
Hspa4L(F2)	TCCCTTTCCCATTACTCTTCG	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa4L(R2)	CACGCACTTTGACCTTCACTTT
Hspa5
Hspa5(F2)	ACCAGCCTACTGTCACTATTA	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa5(R2)	TTGTTCTTGTTGCCTGTG
Hspa8a1
Hspa8a1(F2)	ATGGATAAAGGGCAGATTC	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa8a1(R2)	CCAGGGACAGAGGAGTGA
Hspa8a2
Hspa8a2(F2)	ACTCCTCTGTCCCTGGGTATTG	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa8a2(R2)	CCTTGCTGAGACGACCCTTG
Hspa8b1
Hspa8b1(F2)	GGGGTGCTCATTCAGGTGTT	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa8b1(R2)	TCTGCTTCCTGAACCATACGC
Hspa8b2
Hspa8b2(F2)	GGAGGCTGAGCAAGGAGGAA	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa8b2(R2)	CTGTCCAGCCAGGCAATAACT
Hspa9
Hspa9(F2)	TGTGGGCATACCTGCTAAACG	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa9(R2)	TTGGTAGCCTGAGAATCAAAGAAA
Hspa12a
Hspa12a(F2)	CTCCAACCACAATCCTGCTGAC	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa12a(R2)	GGCTTTGACTCGCTTCCCATT
Hspa12b
Hspa12b(F2)	GCAGCCTGGGTGGACCTAAC	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa12b(R2)	TGGTGATGGTGGGTTGGAAGA
Hspa14
Hspa14(F2)	TGAGCGTGACAGTGCTACAGG	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hspa14(R2)	TCAAAGTCAATGCCGTCGTG
Hprt
Hprt(F)	GAACAGTGACCGCTCCATCC	94°C, 30 s; 58°C, 30 s; 72°C, 30 s; 30cycles
Hprt(R)	TCTTCATCGTCTTTCCCGTGTC