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. 2013 Jun 4;8(6):e65196. doi: 10.1371/journal.pone.0065196

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© 2013 Bryson et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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FITC-dextran (green) facial vein injections and immunostaining for CD31 (red) and cytokeratin (blue) on frozen sagittal sections of fetal mouse thymus. (A–B) FITC-dextran is detected in E14.5 Foxn1^+/Δ thymi, tightly associated with CD31⁺ blood vessels. (C–D) In Foxn1^Δ/Δ embryos, FITC-dextran is also present, but the signal is more diffusely associated with vessels. (E–F) FITC-dextran is present throughout E18.5 Foxn1^+/Δ thymi tightly associated with branched blood vessels. (G–H) FITC-dextran is more diffusely present in the thymus of E18.5 Foxn1^Δ ^/Δ mice. Scale bar, 100 µm; n = 3.