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. 2013 Jun 4;8(6):e65278. doi: 10.1371/journal.pone.0065278

Figure 6. Effects of IMD cotreatment on the LPS-induced gene expression of proinflammatory cytokines in rat testis.

Figure 6

The relative gene expression of TNFα, IL6 and IL1β in the testes 6 h, 12 h, 72 h after LPS treatment or LPS and IMD cotreatment compared with saline control was analyzed by QPCR. (A) Cotreatment of IMD suppressed the LPS-induced increase in TNFα expression at 6 h after treatment. (B) Cotreatment of IMD suppressed the LPS-induced increase in IL6 expression at 12 h after treatment. (C) Cotreatment of IMD suppressed the LPS-induced increase in IL1β expression at 72 h after treatment. Beta-actin served as the reference gene; all data were expressed as mean ± SEM; * P<0.05 by one-way ANOVA followed by Student–Newmann–Keuls test; n = 5–7.